Expression and Localization of Heme Oxygenase in Human Placental Villi
Copyright policy )Expression and Localization of Heme Oxygenase in Human Placental Villi
The aim of the present study was to investigate the expression and distribution patterns of heme oxygenase (HO)-1 and HO-2 in human placental villi at term and in the first trimester of pregnancy using reverse transcription-polymerase chain reaction (RT-PCR), Western blotting, and immunohistochemistry. RT-PCR detected mRNA for HO-1 and HO-2 in human placental villi during gestation. Western blotting also revealed the expression of the two distinct HO proteins throughout gestation. HO-1 was constitutively expressed, while HO-2 expression was apparently greater at term than in early pregnancy. Immunohistochemistry showed that distribution of the two HO isoforms had distinct topographic patterns: HO-1 was observed in villous trophoblastic cells, while HO-2 was found in endothelial cells and smooth muscle cells of blood vessels of placental villi. These results may provide a microtopographic basis for elucidating the mechanism of carbon monoxide (CO)-mediated vasodilatation, and it is suggested that the CO/HO system may be involved in the control of placental vascular function and may protect the syncytiotrophoblast and endothelium against oxidative injury.
Carbon Monoxide, Reverse Transcriptase Polymerase Chain Reaction, Pregnancy Trimester, Third, Blotting, Western, Muscle, Smooth, Immunohistochemistry, Models, Biological, Antibodies, Gene Expression Regulation, Enzymologic, Trophoblasts, Isoenzymes, Pregnancy Trimester, First, Antibody Specificity, Pregnancy, Heme Oxygenase (Decyclizing), Humans, Female, Endothelium, RNA, Messenger, Chorionic Villi
Carbon Monoxide, Reverse Transcriptase Polymerase Chain Reaction, Pregnancy Trimester, Third, Blotting, Western, Muscle, Smooth, Immunohistochemistry, Models, Biological, Antibodies, Gene Expression Regulation, Enzymologic, Trophoblasts, Isoenzymes, Pregnancy Trimester, First, Antibody Specificity, Pregnancy, Heme Oxygenase (Decyclizing), Humans, Female, Endothelium, RNA, Messenger, Chorionic Villi
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).39 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!