Cloning, Genomic Organization, and Tissue Distribution of Human Ssf-1
Copyright policy )Cloning, Genomic Organization, and Tissue Distribution of Human Ssf-1
During the screening of a human placenta cDNA library, realized in order to isolate the P2Y(11) coding sequence, an unrelated cDNA was cloned. We identified a 1422 bp open reading frame encoding a human protein displaying 40% amino acid identity with the Saccharomyces cerevisiae Ssf-1, a protein involved in the second step of mRNA splicing. Sequencing of the corresponding genomic DNA showed that the gene encoding human Ssf-1 is located upstream to the P2Y(11) gene on chromosome 19p31. Comparison of the cDNA and genomic DNA sequences revealed that the human Ssf-1 gene is split into 12 exons. Northern blotting experiments showed that the 1.7 kb Ssf-1 mRNA presents an ubiquitous tissue expression. We also show that, in HL-60 human promyelocytic leukemia cells, Ssf-1 mRNA is rapidly upregulated following a treatment by granulocyte-colony stimulating factor and dibutyryl-cyclicAMP, two agents known to induce the granulocytic differentiation of these cells.
- Université Libre de Bruxelles Belgium
- Erasmus Hospital Belgium
Saccharomyces cerevisiae Proteins, mRNA, Molecular Sequence Data, HL-60 Cells, Nuclear Proteins -- chemistry, Messenger -- genetics, Saccharomyces cerevisiae, Splicing, Chromosomes, Granulocyte Colony-Stimulating Factor -- pharmacology, Saccharomyces cerevisiae -- genetics, Human Ssf-1, Up-Regulation -- drug effects, Granulocyte Colony-Stimulating Factor, Humans, Exons -- genetics, Amino Acid Sequence, RNA, Messenger, Saccharomyces cerevisiae -- chemistry, Cloning, Molecular, Base Sequence, Pair 19 -- genetics, Introns -- genetics, Molecular, Nuclear Proteins, Cell Differentiation, DNA, Exons, Sequence Analysis, DNA, Sciences bio-médicales et agricoles, Yeast, Introns, Up-Regulation, Messenger -- metabolism, HL-60, Cell Differentiation -- drug effects, RNA, Sequence Analysis, Chromosomes, Human, Pair 19, Sequence Alignment, Nuclear Proteins -- genetics, Human, Cloning
Saccharomyces cerevisiae Proteins, mRNA, Molecular Sequence Data, HL-60 Cells, Nuclear Proteins -- chemistry, Messenger -- genetics, Saccharomyces cerevisiae, Splicing, Chromosomes, Granulocyte Colony-Stimulating Factor -- pharmacology, Saccharomyces cerevisiae -- genetics, Human Ssf-1, Up-Regulation -- drug effects, Granulocyte Colony-Stimulating Factor, Humans, Exons -- genetics, Amino Acid Sequence, RNA, Messenger, Saccharomyces cerevisiae -- chemistry, Cloning, Molecular, Base Sequence, Pair 19 -- genetics, Introns -- genetics, Molecular, Nuclear Proteins, Cell Differentiation, DNA, Exons, Sequence Analysis, DNA, Sciences bio-médicales et agricoles, Yeast, Introns, Up-Regulation, Messenger -- metabolism, HL-60, Cell Differentiation -- drug effects, RNA, Sequence Analysis, Chromosomes, Human, Pair 19, Sequence Alignment, Nuclear Proteins -- genetics, Human, Cloning
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