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Biochemical and Biophysical Research Communications
Article . 2000 . Peer-reviewed
License: CC BY NC ND
Data sources: Crossref
Biochemical and Biophysical Research Communications
Article . 2000
Data sources: Europe PubMed Central
https://dx.doi.org/10.1006/bbr...
Article
Data sources: Microsoft Academic Graph
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Solubilization of the Chemokine Receptor CXCR4

descriptionPublicationkeyboard_double_arrow_right Article 01 Jul 2000 English Publisher:Elsevier BVJournal:Biochemical and Biophysical Research Communications, volume 274, pages 153-156 (issn: 0006-291X, Copyright policy )
Authors: R, Staudinger; J C, Bandres;

doi: 10.1006/bbrc.2000.3109

pmid: 10903911

Solubilization of the Chemokine Receptor CXCR4

Abstract

The chemokine receptor CXCR4 was solubilized from the human T-cell line CEM by using the detergent n-dodecyl-beta-maltoside (DDM) and cholesteryl hemisuccinate ester (CHS). Binding studies with (125)I-SDF-1alpha revealed a dissociation constant of 5.33 nM and a receptor density (B(max)) of 2.68 pmol/mg in CEM membranes at 4 degrees C. The affinity of solubilized CXCR4 for SDF-1alpha was identical to membrane-bound CXCR4. Binding of gp120 to solubilized CXCR4 was demonstrated by coprecipitation of gp120 with anti-CXCR4 antibodies.

Related Organizations
Keywords

Receptors, CXCR4, T-Lymphocytes, Cell Membrane, Detergents, HIV Envelope Protein gp120, Precipitin Tests, Chemokine CXCL12, Recombinant Proteins, Cell Line, Iodine Radioisotopes, Kinetics, Glucosides, HIV-1, Humans, Cholesterol Esters, Chemokines, CXC, Protein Binding

  • BIP!
    Impact byBIP!
    selected citations
    These citations are derived from selected sources.
    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    		 15
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    		 Average
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    		 Average
    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
    		 Average
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
15
Average
Average
Average
hybrid