Small Heat Shock Protein 27 (HSP27) Associates with Tubulin/Microtubules in HeLa Cells

descriptionPublicationkeyboard_double_arrow_right Article 01 Apr 2000 English Publisher:Elsevier BVJournal:Biochemical and Biophysical Research Communications, volume 271, pages 164-169 (issn: 0006-291X,

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Authors: M, Hino; K, Kurogi; M A, Okubo; M, Murata-Hori; H, Hosoya;

doi: 10.1006/bbrc.2000.2553

pmid: 10777697

Small Heat Shock Protein 27 (HSP27) Associates with Tubulin/Microtubules in HeLa Cells

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Abstract

One of the monoclonal antibodies raised against mitotic HeLa cells (termed as mH3) recognized a 27-kDa protein and stained microtubules in the mitotic spindles of HeLa cells. Immunoscreening of a HeLa cDNA library revealed that mH3 antigen is a small heat shock protein, HSP27. Immunoprecipitation analysis using mH3 suggested that both alpha- and beta-tubulin are associated with HSP27. Further, sucrose-cushioned ultra centrifugation revealed that HSP27 is co-sedimented with taxol-stabilized microtubules. These results indicate that HSP27 associates with tubulin/microtubules in HeLa cells.

Related Organizations

Hiroshima University
Japan

Keywords

Microscopy, Confocal, Sequence Homology, Amino Acid, Molecular Sequence Data, Antibodies, Monoclonal, Mitosis, Microtubules, Precipitin Tests, Tubulin, Humans, Amino Acid Sequence, Fluorescent Antibody Technique, Indirect, Heat-Shock Proteins, HeLa Cells, Protein Binding

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