Cloning and Expression Study of the Mouse Tetrodotoxin-Resistant Voltage-Gated Sodium Channel α Subunit NaT/Scn11a
Copyright policy )Cloning and Expression Study of the Mouse Tetrodotoxin-Resistant Voltage-Gated Sodium Channel α Subunit NaT/Scn11a
We have cloned a tetrodotoxin-resistant (TTX-R) voltage-gated sodium channel alpha subunit from a mouse cDNA library and designated it as NaT. It encodes 1765 amino acid residues and is virtually identical to that of Scn11a, which has been reported recently, except for 40 nt and 14 aa substitutions. The amino acid identity of NaT/Scn11a with rat NaN/SNS2 is 88%. NaT/Scn11a was mapped to mouse chromosome 9F3-F4 by fluorescence in situ hybridization (FISH). While rat NaN/SNS2 has been reported to be expressed specifically in the peripheral sensory neurons, NaT/Scn11a is expressed not only in the peripheral sensory neurons but also in the spinal cord, uterus, testis, ovary, placenta, and small intestine. NaT is detectable in mouse embryos 15 days postcoitus (p.c.), around the phase of organogenesis and gonadal differentiation. These findings demonstrate a unique distribution of NaT/Scn11a and suggest some of its roles in the above-mentioned processes.
- University of Tokyo Japan
- Osaka University Japan
Male, Reverse Transcriptase Polymerase Chain Reaction, Molecular Sequence Data, Chromosome Mapping, Gene Expression Regulation, Developmental, Recombinant Proteins, Rats, Embryonic and Fetal Development, Mice, Organ Specificity, Pregnancy, Karyotyping, Animals, Female, Amino Acid Sequence, Neurons, Afferent, Peripheral Nerves, Cloning, Molecular, In Situ Hybridization, Fluorescence, Gene Library
Male, Reverse Transcriptase Polymerase Chain Reaction, Molecular Sequence Data, Chromosome Mapping, Gene Expression Regulation, Developmental, Recombinant Proteins, Rats, Embryonic and Fetal Development, Mice, Organ Specificity, Pregnancy, Karyotyping, Animals, Female, Amino Acid Sequence, Neurons, Afferent, Peripheral Nerves, Cloning, Molecular, In Situ Hybridization, Fluorescence, Gene Library
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).12 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!