Expression of a Fungal Cellobiohydrolase in Insect Cells
Copyright policy )Expression of a Fungal Cellobiohydrolase in Insect Cells
The gene for Trichoderma reesei cellobiohydrolase I (CBHI) was expressed with a recombinant baculovirus and high levels of secreted protein were produced in Spodoptera frugiperda and Trichoplusia ni insect cells. Electrophoretic analysis indicated that the recombinant CBHI (rCBHI) was similar in apparent molecular weight to the native form and immunoblotting with anti-CBHI monoclonal antibodies confirmed its identity. The rCBHI was easily purified by affinity and hydrophobic interaction chromatography and demonstrated enzymatic activity on soluble substrate.
- University of Helsinki Finland
- VTT Technical Research Centre of Finland Finland
Trichoderma, Cellulase, Molecular Sequence Data, Cellulose 1,4-beta-Cellobiosidase, Animals, Amino Acid Sequence, Cloning, Molecular, Spodoptera, Cell Line
Trichoderma, Cellulase, Molecular Sequence Data, Cellulose 1,4-beta-Cellobiosidase, Animals, Amino Acid Sequence, Cloning, Molecular, Spodoptera, Cell Line
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