USF2/FIP Associates with the b-Zip Transcription Factor, c-Maf, via Its bHLH Domain and Inhibits c-Maf DNA Binding Activity
Copyright policy )USF2/FIP Associates with the b-Zip Transcription Factor, c-Maf, via Its bHLH Domain and Inhibits c-Maf DNA Binding Activity
In screening for proteins that interact with the basic zipper (bZip) transcription factor, c-Maf, we isolated USF2/FIP. USF2 is a member of the bHLH-Zip protein family, possessing a basic (b) DNA binding region, a helix-loop-helix (HLH) motif, and a leucine zipper (Zip) structure. Mutants of USF2 that lacked a Zip formed heterodimers with c-Maf, but did not homodimerize. Deletion of the USF2 basic region or mutation of its helices abrogated its binding to c-Maf, but had no effect on homodimerization. A functional c-Maf bZip motif was necessary for both homodimerization and heterodimerization with USF2. These data suggest a tetrameric configuration for Maf-USF2 complexes. In the presence of USF2, the DNA binding activity of c-Maf was markedly reduced. Therefore, USF2 and c-Maf may interact to regulate gene expression.
- St. Jude Children's Research Hospital United States
Leucine Zippers, Binding Sites, Kainic Acid, Helix-Loop-Helix Motifs, Nerve Tissue Proteins, Recombinant Proteins, DNA-Binding Proteins, Basic-Leucine Zipper Transcription Factors, Biopolymers, G-Box Binding Factors, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-maf, Upstream Stimulatory Factors, Cycloheximide, Promoter Regions, Genetic, Protein Binding, Transcription Factors
Leucine Zippers, Binding Sites, Kainic Acid, Helix-Loop-Helix Motifs, Nerve Tissue Proteins, Recombinant Proteins, DNA-Binding Proteins, Basic-Leucine Zipper Transcription Factors, Biopolymers, G-Box Binding Factors, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-maf, Upstream Stimulatory Factors, Cycloheximide, Promoter Regions, Genetic, Protein Binding, Transcription Factors
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).25 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!