Hexose Recognition by Insulin-Secreting BRIN-BD11 Cells

descriptionPublicationkeyboard_double_arrow_right Article 01 Jun 1996 English Publisher:Elsevier BVJournal:Biochemical and Biophysical Research Communications, volume 223, pages 724-728 (issn: 0006-291X,

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Authors: N H, McClenaghan; A M, Gray; C R, Barnett; P R, Flatt;

doi: 10.1006/bbrc.1996.0963

pmid: 8687464

Hexose Recognition by Insulin-Secreting BRIN-BD11 Cells

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Abstract

Clonal BRIN-BD11 cells were produced by electrofusion of NEDH rat islet B-cells with immortal RINm5F cells. Western blotting analysis revealed that unlike RINm5F, novel BRIN-BD11 cells expressed high levels of the glucose transport protein GLUT-2, coupled with a rapid and sustained uptake of D-glucose, significantly greater than RINm5F after only 5 min (p < 0.05). Whereas BRIN-BD11 cells expressed a high glucokinase:hexokinase ratio with 1.4-2.0 fold and 1.4-1.7 fold stepwise stimulation of insulin secretion with 4.2-16.7 mM D-glucose and D-mannose respectively, RINm5F had a lower glucokinase:hexokinase ratio (p < 0.001) and were notably unresponsive to D-glucose and D-mannose. Unlike RINm5F cells, BRIN-BD11 were unresponsive to other hexoses, with RINm5F only responding to D-galactose (p < 0.05). BRIN-BD11 cells should be useful for studies of nutrient-induced insulin secretion.

Related Organizations

University of Ulster
United Kingdom

Keywords

Deoxyglucose, Cell Line, Clone Cells, Rats, Cell Fusion, Pancreatic Neoplasms, Islets of Langerhans, Kinetics, Glucose, Hexokinase, Glucokinase, Insulin Secretion, Animals, Insulin, Insulinoma, Mannose, Hexoses

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