Purification and Characterization of a Glutathione Dependent Dehydroascorbate Reductase from Human Erythrocytes

descriptionPublicationkeyboard_double_arrow_right Article 01 Apr 1996 English Publisher:Elsevier BVJournal:Biochemical and Biophysical Research Communications, volume 221, pages 117-121 (issn: 0006-291X,

Copyright policy )

Authors: D P, Xu; M P, Washburn; G P, Sun; W W, Wells;

doi: 10.1006/bbrc.1996.0555

pmid: 8660320

Purification and Characterization of a Glutathione Dependent Dehydroascorbate Reductase from Human Erythrocytes

- Summary
- Subjects
- Metrics

Abstract

A GSH-dependent dehydroascorbate reductase (EC 1.8.5.1) was purified to homogeneity from human erythrocytes. The enzyme was a monomer of 32 kDa and was purified 133-fold from a crude DEAE-Sepharose fraction with a 25% yield. The reduced protein had a pI of 5.1 as judged by isoelectric focusing. Kinetic analysis gave a Kcat of 316 min-1, a Km of 0.21 mM for DHA with a Kcat/Km of 2.47 x 10(4) M-1 sec-1, and a Km of 3.5 mM for GSH with a Kcat/Km of 1.51 x 10(3) M-1 sec-1. This is the second DHA reductase (after thioltransferase) isolated from human erythrocytes, but unlike thioltransferase, it has no thiol-disulfide oxido-reductase activity.

Related Organizations

Michigan State University
United States

Keywords

Kinetics, Erythrocytes, Humans, Electrophoresis, Polyacrylamide Gel, Protein Disulfide Reductase (Glutathione), Isoelectric Focusing, Chromatography, Ion Exchange, Oxidoreductases, Glutathione, Glutaredoxins

Impact byBIP!

	selected citations These citations are derived from selected sources. This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	59
	popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.	Top 10%
	influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	Top 10%
	impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.	Top 10%