Characterization of Human Prodynorphin Gene Transcripts
Copyright policy )Characterization of Human Prodynorphin Gene Transcripts
Prodynorphin gene transcripts have been characterized in human tissues with cRNA probes covering parts of the non-coding exon 1 and the main coding exon 4 using Northern blot and RNase protection experiments. A 2.8-kb signal was observed in human brain RNA with both the exon 1 and exon 4 probes. An RNase protection assay with the exon 1 probe, performed to map the 5' end of this transcript, produced protected fragments in the range of 0.11 to 0.15 kb indicating that exon 1 is 1.2 kb shorter than previously proposed. 5'-RACE-PCR and sequencing of amplified cDNA fragments confirmed this assignment. In adrenal gland, testis and the human small cell lung carcinoma cell line, U1690, several prodynorphin-like mRNAs structurally different from the brain prodynorphin mRNA species were observed.
- Karolinska Institute Sweden
Male, Lung Neoplasms, Base Sequence, Transcription, Genetic, Molecular Sequence Data, Brain, Gene Expression, Enkephalins, Exons, RNA Probes, Polymerase Chain Reaction, Cell Line, Organ Specificity, Protein Biosynthesis, Adrenal Glands, Testis, Humans, RNA, Messenger, Carcinoma, Small Cell, Protein Precursors
Male, Lung Neoplasms, Base Sequence, Transcription, Genetic, Molecular Sequence Data, Brain, Gene Expression, Enkephalins, Exons, RNA Probes, Polymerase Chain Reaction, Cell Line, Organ Specificity, Protein Biosynthesis, Adrenal Glands, Testis, Humans, RNA, Messenger, Carcinoma, Small Cell, Protein Precursors
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