Secretion Kinetics of Alzheimer′s Amyloid β-Protein Differs from Secreted β-Amyloid Precursor Protein
Copyright policy )Secretion Kinetics of Alzheimer′s Amyloid β-Protein Differs from Secreted β-Amyloid Precursor Protein
Amyloid beta-protein (A beta) and secreted beta-amyloid precursor protein (sAPP), derived from beta-amyloid precursor protein (APP), are normally released by cultured mammalian cells. We investigated by pulse-chase analysis the secretion kinetics of these two APP derivatives using mouse cholinergic SN49 cell lines stably transfected with mouse APP695 cDNA. After both A beta and sAPP peaked at about the second hour, sAPP decreased with a half-life of approximately 5 hours, but A beta remained almost unchanged for at least 14 hours. These results indicate that A beta is more stable than sAPP in the SN49 conditioned medium.
Amyloid beta-Protein Precursor, Kinetics, Mice, Amyloid beta-Peptides, Methionine, Animals, Autoradiography, Sulfur Radioisotopes, Transfection, Recombinant Proteins, Cell Line
Amyloid beta-Protein Precursor, Kinetics, Mice, Amyloid beta-Peptides, Methionine, Animals, Autoradiography, Sulfur Radioisotopes, Transfection, Recombinant Proteins, Cell Line
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