Identification and Cloning of a Second Phytase Gene (phyB) from Aspergillus niger (ficuum)
Copyright policy )Identification and Cloning of a Second Phytase Gene (phyB) from Aspergillus niger (ficuum)
An Aspergillus niger (ficuum) genomic DNA lambda EMBL3 library was probed with a 354-bp DNA fragment obtained by polymerase chain reaction of A. niger DNA with oligonucleotides based on partial amino acid sequence of a pH 2.5 optimum acid phosphatase. A clone containing a 1605 bp segment (phyB) encoding the 479 amino acid enzyme was isolated and found to contain four exons. Global alignment revealed 23.5% homology to Aspergillus niger phytase (PhyA); four regions of extensive homology were identified. Some of these regions may contain catalytic sites for phosphatase function.
- United States Department of Agriculture United States
- Agricultural Research Service United States
- Southern Regional Research Center United States
6-Phytase, Base Sequence, Sequence Homology, Amino Acid, Genes, Fungal, Molecular Sequence Data, Polymerase Chain Reaction, Isoenzymes, Oligodeoxyribonucleotides, Amino Acid Sequence, Aspergillus niger, Cloning, Molecular, DNA, Fungal, Software
6-Phytase, Base Sequence, Sequence Homology, Amino Acid, Genes, Fungal, Molecular Sequence Data, Polymerase Chain Reaction, Isoenzymes, Oligodeoxyribonucleotides, Amino Acid Sequence, Aspergillus niger, Cloning, Molecular, DNA, Fungal, Software
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