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Monoclonal Antibodies against Human Neurotrophin-3

descriptionPublicationkeyboard_double_arrow_right Article 01 Aug 1993Publisher:Elsevier BVJournal:Biochemical and Biophysical Research Communications, volume 194, pages 1,500-1,507 (issn: 0006-291X,

Authors: K. Kuroshima; Yuzo Ichimori; Takuya Watanabe; Reiko Sasada; Tsutomu Kurokawa; Koichi Igarashi; Asae Shintani;

doi: 10.1006/bbrc.1993.1994

pmid: 8352808

Monoclonal Antibodies against Human Neurotrophin-3

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Abstract

Hybridomas producing monoclonal antibodies (MoAbs) against human neurotrophin-3 (hNT-3) were established using recombinant hNT-3 produced in CHO cells and E. coli as immunogens. Of the five MoAbs obtained, MoAb 3w3 showed the highest antibody titer and also best neutralized NT-3 activity as measured by the survival of chick embryonic day-8 dorsal root ganglia neurons. A sandwich enzyme immunoassay (EIA) for NT-3 was established with solid phase MoAb 3W3 and the Fab' fragment of MoAb 3W3 conjugated to horseradish peroxidase. The detection limit was 2.7 pg/well of NT-3 and no cross-reactivity with nerve growth factor up to 100 ng/well was observed. Using this EIA system we have screened a variety of cell lines for NT-3 production. Among these tested, only human Burkitt's lymphoma Namalwa cells were found to be producing NT-3.

Related Organizations

Takeda (Japan)
Japan

Keywords

Neurons, Hybridomas, Cell Survival, Antibodies, Monoclonal, CHO Cells, Chick Embryo, Burkitt Lymphoma, Recombinant Proteins, Immunoenzyme Techniques, Neurotrophin 3, Neutralization Tests, Cricetinae, Ganglia, Spinal, Escherichia coli, Animals, Humans, Nerve Growth Factors

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