Isolation and characterization of the gene URA3 encoding the orotidine‐5′‐phosphate decarboxylase from Torulaspora delbrueckii
Copyright policy )Isolation and characterization of the gene URA3 encoding the orotidine‐5′‐phosphate decarboxylase from Torulaspora delbrueckii
AbstractA DNA fragment containing the URA3 gene from Torulaspora delbrueckii was isolated by complementation cloning in Saccharomyces cerevisiae. DNA sequence analysis revealed the presence of an ORF of 795 bp, encoding a 264 amino acid protein, which shares a high similarity to the Saccharomycetaceae Ura3 proteins. Furthermore, the cloned ORF fully complemented the ura3 mutation of S. cerevisiae, confirming that it encodes for the TdUra3 protein. The GeneBank Accession No. for TdURA3 is AF518402. Copyright © 2002 John Wiley & Sons, Ltd.
Fungal Proteins, Open Reading Frames, Base Sequence, Sequence Homology, Amino Acid, Yeasts, Genes, Fungal, Molecular Sequence Data, Orotidine-5'-Phosphate Decarboxylase, Amino Acid Sequence
Fungal Proteins, Open Reading Frames, Base Sequence, Sequence Homology, Amino Acid, Yeasts, Genes, Fungal, Molecular Sequence Data, Orotidine-5'-Phosphate Decarboxylase, Amino Acid Sequence
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