The nucleotide sequence and initial characterization of pyruvate decarboxylase from the yeast Hanseniaspora uvarum
Copyright policy )The nucleotide sequence and initial characterization of pyruvate decarboxylase from the yeast Hanseniaspora uvarum
AbstractWe have isolated a pyruvate decarboxylase (PDC) gene from the yeast Hanseniaspora uvarum using the Saccharomyces cerevisiae PDC1 gene as a probe. The nucleotide sequence of this gene was determined and compared to PDC genes from yeast and other organisms. The H. uvarum PDC gene is more than 70% identical to the S. cerevisiae PDC isozymes and possesses a putative thiamine diphosphate binding site. The PDC enzyme was purified and partially characterized. The H. uvarum PDC was very similar to other known PDCs; the Km for pyruvate was 0·75 mM, and the enzyme is a homotetramer with subunits of Mr = 57 000. The sequence has been submitted to GenBank under Accession No. U13635.
- University of Guelph Canada
Molecular Weight, Base Sequence, Molecular Sequence Data, Saccharomycetales, Amino Acid Sequence, Hydrogen-Ion Concentration, Pyruvate Decarboxylase
Molecular Weight, Base Sequence, Molecular Sequence Data, Saccharomycetales, Amino Acid Sequence, Hydrogen-Ion Concentration, Pyruvate Decarboxylase
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