Isolation and characterization of Candida kefyr orotidine‐5′‐phosphate decarboxylase (URA3) gene
Copyright policy )Isolation and characterization of Candida kefyr orotidine‐5′‐phosphate decarboxylase (URA3) gene
AbstractCandida kefyr is a common yeast species that can be found in fermented milk and cheeses. As a first step to developing a gene transfer system for C. kefyr, the orotidine‐5′‐phosphate decarboxylase (URA3) gene was cloned, using degenerate PCR and genome walking. The uninterrupted open reading frame of the C. kefyr URA3 gene spans 801 bp, corresponding to 267 amino acid residues. The functionality of the gene was confirmed by complementation of ura3 auxotrophs of C. albicans and Saccharomyces cerevisiae. Phylogenetic analysis of the deduced amino acid sequence indicated that it shares a high degree of homology with other Candida URA3 homologues. The GenBank Accession No. of the C. kefyr URA3 gene is FJ914763. Copyright © 2009 John Wiley & Sons, Ltd.
- University of Hong Kong (香港大學) China (People's Republic of)
- Chinese University of Hong Kong China (People's Republic of)
- University of Hong Kong China (People's Republic of)
Orotidine-5'-Phosphate Decarboxylase - Genetics - Metabolism, Orotidine-5'-Phosphate Decarboxylase, Enzymologic - Physiology, Molecular, Fungal - Physiology, Fungal Proteins - Genetics - Metabolism, Gene Expression Regulation, Enzymologic - Physiology, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Fungal - Physiology, Fungal Proteins, Gene Expression Regulation, Gene Expression Regulation, Fungal, Amino Acid Sequence, Cloning, Molecular, Candida - Enzymology, Phylogeny, Cloning, Candida
Orotidine-5'-Phosphate Decarboxylase - Genetics - Metabolism, Orotidine-5'-Phosphate Decarboxylase, Enzymologic - Physiology, Molecular, Fungal - Physiology, Fungal Proteins - Genetics - Metabolism, Gene Expression Regulation, Enzymologic - Physiology, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Fungal - Physiology, Fungal Proteins, Gene Expression Regulation, Gene Expression Regulation, Fungal, Amino Acid Sequence, Cloning, Molecular, Candida - Enzymology, Phylogeny, Cloning, Candida
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