ABSTRACTAdenosine‐to‐inosine (A‐to‐I) RNA editing is a key post‐transcriptional modification that influences gene expression and various cellular processes. Advances in sequencing technologies have greatly contributed to the identification of A‐to‐I editing sites, providing insights into their distribution across coding and non‐coding regions. These developments have facilitated the discovery of functionally relevant editing events and have advanced the understanding of their biological roles. This review presents the evolution of methodologies for RNA editing detection and examines recent advances, including chemically‐assisted, enzyme‐assisted, and quantitative approaches. By evaluating these techniques, we aim to help researchers select the most effective tools for investigating RNA editing and its broader implications in health and disease.