Microfluidic Single-Cell Analysis with Affinity Beads

descriptionPublicationkeyboard_double_arrow_right Article , Conference object , Other literature type 29 Jan 2015 Switzerland English Publisher:WileyJournal:Small, volume 11, pages 3,511-3,511 (issn: 1613-6810,

Copyright policy )Funded by:EC | SYNSIGNAL

Authors: Michael Werner; Raghavendra Palankar; Loïc Arm; Ruud Hovius; Horst Vogel;

doi: 10.1002/smll.201501535 , 10.1002/smll.201402650

pmid: 25641862

handle: 20.500.14299/110688 , 20.500.14299/103947

Microfluidic Single-Cell Analysis with Affinity Beads

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Abstract

A micrometer-sized affinity bead (red) is (i) taken up into a cell by phagocytosis, (ii) photochemically released from phagosomes, (iii) optically trapped by the cell, and (iv) isolated by cell lysis for subsequent analysis of captured intracellular analyte (green).

Country

Switzerland

Related Organizations

École Polytechnique Fédérale de Lausanne EPFL
Switzerland

Keywords

Immunoassay, Optical Tweezers, optical tweezers, photochemical internalization, Microfluidics, microfluidics, Cell Separation, Flow Cytometry, affinity beads, HEK293 Cells, single-cell analysis, Humans

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