A laboratory study was conducted to determine the feasibility of biotreatment of liquid Sulfinol waste stored at a gas treatment facility. Sulfinol is used to scrub impurities from gas generated from both traditional gas extraction and that from hydraulic fracturing processes. Chemical characterization and microbiological assessment showed that cultures of Sulfinol-degrading microorganisms could be enriched from Sulfinol-contaminated soil containing saturated concentrations of Sulfinol: a mixture of di-isopropanolamine (DiPA), sulfolane, and oxazolidone. Based on this initial finding from the enrichment culture study, batch reactors were incubated with inoculants from enrichment cultures containing known numbers of presumptive Sulfinol-degrading microorganisms. The microbial analyses of liquors from batch reactors showed microbial inhibition and/or loss of viability due to Sulfinol toxicity, even at the lowest Sufinol waste concentration used (5 percent of the original waste). The changes in concentrations of the chemicals in the batch reactor trials were a result of chemical rather than biodegradation processes. Further research is recommended to develop repeatable strategies for biodegrading the constituents of Sulfinol under field conditions.