AbstractHere we report the synthesis of the N‐terminal hexapeptide H‐Pro‐Arg‐Arg‐Arg‐Val‐Ile‐OH of the E. coli ribosomal protein S7, the C‐terminal hexapeptide H‐Lys‐Glu‐Ala‐Lys‐Lys‐Lys‐OH of L6 and the C‐terminal hexapeptide H‐Pro‐Gln‐Val‐Leu‐Asp‐Ile‐OH of L13. All peptides were prepared by SPPS following the Fmoc‐strategy, using DIC/HOBt and/or HBTU as coupling reagents and 2‐chlorotrityl chloride resin as the solid support. The carrier linked synthetic peptides were injected into rabbits and elicited an anti‐peptide response. These anti‐hexapeptide antibodies were found to recognize the corresponding peptides and proteins. Copyright © 2002 European Peptide Society and John Wiley & Sons, Ltd.