Abstract BACKGROUND Autophagy plays an important role against pathogen infections in both insects and plants. Insect vectors employ autophagy as an intrinsic antiviral defense mechanism against viral infections, whereas viruses can exploit autophagy to enhance their transmission via insect vectors. The Cotton leaf curl Multan virus (CLCuMuV) is transmitted by the AsiaII7 cryptic species of Bemisia tabaci , however, the role of autophagy is involved in regulating the transmission of this virus remains unclear. RESULT In this study, it was observed that CLCuMuV infection induced autophagy in AsiaII7 whitefly, as evidenced by an elevated in the level of ATG8‐II and the upregulation of Atg3, Atg8 , Atg9 and Atg12 . Both the administration of the autophagy inhibitor bafilomycin A1 and the silencing of Atg9 expression increased the viral load and enhanced CLCuMuV transmission. Conversely, the activation of autophagy via rapamycin feeding significantly reduced the amount of CLCuMuV and inhibited the efficiency of virus transmission. CONCLUSION CLCuMuV infection can activate the autophagy pathway in whiteflies. The activation of autophagy leads to the subsequent degradation of the virus and suppresses CLCuMuV transmission efficiency, whereas suppression of autophagy promotes virus transmission. Our research provides insight into the potential role of autophagy in antiviral defense mechanisms. © 2025 Society of Chemical Industry.