Abstract BACKGROUND Polypogon fugax is a problematic annual grass weed that severely threatens oilseed rape and wheat production by competing for essential resources and reducing crop yields. Haloxyfop‐R‐methyl has been widely used for its effective control; however, its intensive and prolonged application has driven the rapid evolution of resistance in P. fugax populations. RESULTS In this study, a CYP94 family gene, designated CYP94D‐2 , was identified from a previously characterized haloxyfop‐R‐methyl‐resistant P. fugax population and showed markedly elevated expression. Functional analysis revealed that rice plants overexpressing CYP94D‐2 showed significantly reduced growth inhibition after haloxyfop‐R‐methyl treatment, accompanied by enhanced herbicide‐metabolizing capacity. Molecular docking analysis further supported a strong interaction between CYP94D‐2 and haloxyfop‐R‐methyl, consistent with its potential to catalyze efficient herbicide metabolism. CONCLUSION These results demonstrate that CYP94D‐2 is involved in haloxyfop‐R‐methyl resistance via a metabolism‐based mechanism. This study expands our understanding of the CYP94 family's role in herbicide resistance and provides new insights into the molecular basis of non‐target‐site resistance in weeds. © 2026 Society of Chemical Industry.