AbstractBACKGROUNDSpodoptera eridania (Stoll), S. cosmioides (Walker) and S. albula (Walker) (Lepidoptera: Noctuidae) are considered secondary pests of soybean in South America. The genetically‐modified soybean DAS‐444Ø6‐6 × DAS‐81419‐2 with tolerance to 2,4‐dichlorophenoxyacetic acid (2,4‐D), glyphosate and ammonium glufosinate herbicides (event DAS‐444Ø6‐6) and insect‐resistance due to expression of Cry1Ac and Cry1F Bt proteins (event DAS‐81419‐2) may provide a potential tool for integrated pest management (IPM) of these species in soybean fields. Based on this, we conducted bioassays to evaluate the survival and development of S. eridania, S. cosmioides and S. albula fed on Cry1Ac/Cry1F‐soybean leaf tissue.RESULTSSpodoptera eridania and S. cosmioides fed on Cry1Ac/Cry1F‐soybean showed longer developmental time, lower larval and egg to adult survival compared to those fed on non‐Bt soybean, reducing the population growth of these species. Spodoptera albula also had lower larval survival and number of insects that reached adulthood on Cry1Ac/Cry1F‐soybean. However, no significant effects of Cry1Ac/Cry1F‐soybean on population growth parameters were detected in this species.CONCLUSIONSSoybean with stacked events DAS‐444Ø6‐6 × DAS‐81419‐2 expressing Cry1Ac/Cry1F Bt proteins provide population suppression of S. eridania and S. cosmioides. However, this Bt soybean had minimal effects on S. albula, and is unlikely to have negative population‐level effects on this species. It is expected that under field conditions, other control tactics must be integrated with Cry1Ac/Cry1F‐soybean for the management of these Spodoptera species. © 2020 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.