AbstractObjectiveThe aim of this study was to survey the opinions of expectant parents regarding prenatal whole‐exome sequencing.MethodsThe study used a questionnaire that focused on acceptability of prenatal whole‐exome sequencing to individuals who pursued first‐trimester prenatal screening in a tertiary academic medical center. A total of 186 expectant individuals completed the questionnaire. The results of the questionnaire were analyzed using descriptive statistics and logistic regression models.ResultsEighty‐three percent of the participants answered that prenatal whole‐exome sequencing should be offered, 14.8% were neutral, and only 2.2% disagreed. Fifty‐four percent of the participants were interested in having prenatal whole‐exome sequencing for their fetus, 40.1% were neutral, and 6.6% disagreed. The majority of participants expressed a desire to know about treatable (96.2%) and non‐treatable (86.3%) childhood conditions, and most said the same for treatable (76.0%) and non‐treatable (74.3%) adult‐onset conditions. Over half of the participants (59.7%) indicated a maximum acceptable turnaround time of 3 weeks or less for prenatal whole‐exome sequencing.ConclusionsThe majority of respondents felt prenatal whole‐exome sequencing should be offered. Moreover, the majority wanted to know prenatally about treatable and non‐treatable childhood and adult conditions. © 2015 John Wiley & Sons, Ltd.