PIN1 expression contributes to hepatic carcinogenesis
Copyright policy )PIN1 expression contributes to hepatic carcinogenesis
AbstractThe phospho‐Ser/Thr‐Pro specific prolyl‐isomerase PIN1 is over‐expressed in more than 50% of hepatocellular carcinomas (HCCs). To investigate its potential oncogenicity, we over‐expressed PIN1 in a non‐transformed human liver cell line MIHA. This resulted in up‐regulation of β‐catenin and cyclin D1, leading to anchorage‐independent growth in soft agar and tumorigenicity in nude mice. To further validate the role of PIN1 in hepatocarcinogenesis, PIN was suppressed by RNA interference (siRNA) in the HCC cell line PLC/PRF/5. siRNA‐PIN1 transfection of PLC/PRF/5 cells led to repression of PIN1 expression, resulting in decreased levels of β‐catenin and cyclin D1. siRNA‐PIN1 transfectants showed lower cell proliferation rates, reduced colony formation, and retarded cell cycle progression, with an increase in cells residing in G0/G1. Furthermore, soft agar colony formation was depressed, and tumorigenicity in nude mice was abrogated. These findings implicate PIN1 expression as an important step in hepatic carcinogenesis. Copyright © 2006 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
- Queen Mary Hospital China (People's Republic of)
- Hospital Authority China (People's Republic of)
- University of Hong Kong China (People's Republic of)
- University of Hong Kong (香港大學) China (People's Republic of)
Cyclin D1 - genetics, Neoplasm - genetics, Carcinoma, Hepatocellular, Hepatocellular carcinoma, Nude, Mice, Nude, Neoplasm Proteins - genetics, Transfection, Cell Line, Mice, PIN1, RNA interference, Cell Line, Tumor, Animals, Humans, Cyclin D1, RNA, Neoplasm, beta Catenin, Tumor, Carcinoma, Liver Neoplasms - genetics, Liver Neoplasms, Up-Regulation - genetics, Peptidylprolyl Isomerase - genetics, Transfection - methods, Peptidyl-prolyl-isomerase, β-catenin, Carcinoma, Hepatocellular - genetics, Peptidylprolyl Isomerase, beta Catenin - genetics, RNA, Neoplasm - genetics, Neoplastic - genetics, Neoplasm Proteins, Up-Regulation, Gene Expression Regulation, Neoplastic, NIMA-Interacting Peptidylprolyl Isomerase, Gene Expression Regulation, Hepatocellular - genetics, RNA, Gene Expression Regulation, Neoplastic - genetics, RNA Interference
Cyclin D1 - genetics, Neoplasm - genetics, Carcinoma, Hepatocellular, Hepatocellular carcinoma, Nude, Mice, Nude, Neoplasm Proteins - genetics, Transfection, Cell Line, Mice, PIN1, RNA interference, Cell Line, Tumor, Animals, Humans, Cyclin D1, RNA, Neoplasm, beta Catenin, Tumor, Carcinoma, Liver Neoplasms - genetics, Liver Neoplasms, Up-Regulation - genetics, Peptidylprolyl Isomerase - genetics, Transfection - methods, Peptidyl-prolyl-isomerase, β-catenin, Carcinoma, Hepatocellular - genetics, Peptidylprolyl Isomerase, beta Catenin - genetics, RNA, Neoplasm - genetics, Neoplastic - genetics, Neoplasm Proteins, Up-Regulation, Gene Expression Regulation, Neoplastic, NIMA-Interacting Peptidylprolyl Isomerase, Gene Expression Regulation, Hepatocellular - genetics, RNA, Gene Expression Regulation, Neoplastic - genetics, RNA Interference
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