AbstractWe constructed a device to compress small samples of articular cartilage while the samples were imaged in a 1.5 T imager. With the use of a piezoelectric piston, the device compressed 1‐cm‐diameter cylindrical samples of articular cartilage (200 μm) at a rate of 2 Hz. Simultaneously, we imaged the samples with a displacement‐sensitive stimulated‐echo acquisition mode (STEAM) sequence. We validated the technique using tissue that mimicked silicone samples. We compared the results from the same cartilage samples before and after they were degraded by digestion in trypsin. The extent of degradation was visualized from T1‐weighted images of the samples after they were soaked in 0.5 mmolar of GdDTPA. The resulting elastographic images show compression and differential strain in directions both parallel and perpendicular to the surface of the cartilage. The static elastographic images that depict compression made before digestion and after 5 and 15 hr of trypsin digestion show that the elastic modulus of the samples decreased with a spatial variation consistent with the enzymatic digestion as revealed by the T1 images. We believe this technique will be useful in studies of the mechanical properties of articular cartilage and other tissues, and may in the future be extended to the clinical setting. Magn Reson Med 53:1065–1073, 2005. © 2005 Wiley‐Liss, Inc.