Rabbit blastocysts accumulate platelet‐activating factor (PAF) and lyso‐PAF in vitro
Copyright policy )Rabbit blastocysts accumulate platelet‐activating factor (PAF) and lyso‐PAF in vitro
AbstractPlatelet‐activating factor (1‐O‐alkyl‐2‐acetyl‐sn‐glycero‐3‐phosphorylcholine; PAF) is a very potent phospholipid, which has been demonstrated to stimulate smooth muscle and change vascular permeability. PAF has been detected in the rabbit preimplantation uterine endometrium and has been demonstrated to bind specifically to rabbit uterine membranes. To evaluate the possible role of PAF in maternal‐embryonic chemical communication, we report here that rabbit blastocysts can accumulate [3H]PAF from their environment. Blastocysts were able to accumulate [3H]PAF as time‐, buffer‐, age‐, and concentration‐dependent functions. The accumulation was inhibited by some PAF receptor antagonists, such as U66985, as well as by unlabeled PAF and lyso‐PAF, indicating that the accumulation process may be receptor mediated. The data support the current model of PAF as a paracrine factor in preimplantation stages of reproduction.
- The University of Texas Health Science Center at San Antonio United States
- Illinois State University United States
Embryonic Development, Receptors, Cell Surface, Platelet Membrane Glycoproteins, Receptors, G-Protein-Coupled, Blastocyst, Organ Culture Techniques, Pregnancy, Animals, Female, Rabbits, Platelet Activating Factor, Maternal-Fetal Exchange
Embryonic Development, Receptors, Cell Surface, Platelet Membrane Glycoproteins, Receptors, G-Protein-Coupled, Blastocyst, Organ Culture Techniques, Pregnancy, Animals, Female, Rabbits, Platelet Activating Factor, Maternal-Fetal Exchange
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).3 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average
Citations provided by BIP!Popularity provided by BIP!