Immunological evidence for progesterone and estradiol receptors in the freshwater crayfishAustropotamobius pallipes
Copyright policy )Immunological evidence for progesterone and estradiol receptors in the freshwater crayfishAustropotamobius pallipes
AbstractIn this article, we present evidence for progesterone and estradiol receptors (PR and ER, respectively) in the female of the crayfishAustropotamobius pallipes. To our knowledge, this is the first report of sex steroid receptors in crustaceans. By using immunohistochemistry and Western blotting approaches and employing three different antibodies against PR (human PR, chicken PR‐hinge region, and chicken PR A/B domain) and antibodies against human ER, we showed the presence of PR in the ovary and hepatopancreas and ER in the hepatopancreas of the freshwater crayfishA. pallipes. The immunological characteristics and the tissue localization suggest a relatedness with both PR and ER in vertebrates along with their involvement in the modulation of reproductive functions in this crustaceans. Mol. Reprod. Dev. 63: 55–62, 2002. © 2002 Wiley‐Liss, Inc.
- University Federico II of Naples Italy
- University of Sannio Italy
Male, Blotting, Western, Ovary, Astacoidea, Receptors, Estradiol, Immunoenzyme Techniques, Liver, Organ Specificity, Animals, Female, Receptors, Progesterone, Pancreas
Male, Blotting, Western, Ovary, Astacoidea, Receptors, Estradiol, Immunoenzyme Techniques, Liver, Organ Specificity, Animals, Female, Receptors, Progesterone, Pancreas
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