ABSTRACT Amblyopia is a neurodevelopmental disorder characterized by impaired vision in childhood and remains challenging to manage effectively. The present study investigated the neuroprotective potential of Rubus idaeus L. (red raspberry) in a rodent model of amblyopia. Through network pharmacology analysis, the PI3K/AKT axis was identified as a major regulatory target of R. idaeus L. treatment. In vitro, 1 µg/mL of R. idaeus L. significantly enhanced neuronal survival under oxidative stress (82.4% vs. 54.6%, p < 0.01) and reduced apoptotic cell death by 23.5% ( p < 0.001). These protective effects correlated with elevated expression of anti‐apoptotic proteins (Bcl‐2, PI3K) and decreased levels of pro‐apoptotic markers (Bax, cleaved caspase‐3). In vivo, R. idaeus L. treatment improved visual evoked potentials in a monocular deprivation model, indicated by shorter P 100 latency and increased amplitude. These effects were reversed by the PI3K inhibitor LY294002, supporting the involvement of the PI3K/AKT axis. Our findings suggest that R. idaeus L. holds potential as a novel therapeutic approach for amblyopia via modulation of neuroprotective pathways.