Analysis of CYP1A1 promoter function by transcription in vitro
Copyright policy )Analysis of CYP1A1 promoter function by transcription in vitro
AbstractWe have analyzed the function of the CYP1A1 promoter using in vitro transcription. Using nuclear extracts from HeLa cells, we found that transcription from the promoter in vitro was constitutive. Transcription in vitro was not increased by prior exposure of the HeLa cells to the inducer 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin nor by the inclusion of a dioxin‐responsive enhancer in the DNA template. Analyses of mutants revealed that a TATAAA sequence and two recognition motifs for CCAAT box transcription factor/nuclear factor I contributed to the constitutive activity of the promoter in vitro.
- Stanford University United States
Cell Nucleus, Polychlorinated Dibenzodioxins, Base Sequence, Cytochrome P-450 Enzyme System, Transcription, Genetic, Humans, Templates, Genetic, Promoter Regions, Genetic, TATA Box, HeLa Cells
Cell Nucleus, Polychlorinated Dibenzodioxins, Base Sequence, Cytochrome P-450 Enzyme System, Transcription, Genetic, Humans, Templates, Genetic, Promoter Regions, Genetic, TATA Box, HeLa Cells
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