Human S15a expression is upregulated by hepatitis B virus X protein
Copyright policy )Human S15a expression is upregulated by hepatitis B virus X protein
AbstractThe hepatitis B virus (HBV)–encoded X antigen (HBxAg) may contribute to the development of hepatocellular carcinoma (HCC) through the upregulated expression of selected cellular genes. To identify these genes, RNAs isolated from HBxAg‐positive and ‐negative HepG2 cells were compared by PCR select cDNA subtraction. One gene overexpressed in HBxAg‐positive cells by Northern and Western blotting is the ribosomal protein S15a. The S15a mRNA is 535 base pairs, encoding a protein 130 amino acids long with a molecular weight of 14.3 kDa. S15a expression was upregulated in HBV‐infected livers, where it costained with HBxAg. Overexpression of S15a stimulated cell growth, colony formation in soft agar, and tumor formation in SCID mice. Hence, HBxAg upregulated the expression of S15a, the latter of which participates in the development of HCC, perhaps by altering the integrity of translation. © 2004 Wiley‐Liss, Inc.
- Second Military Medical University China (People's Republic of)
- Changhai Hospital China (People's Republic of)
- Pamukkale University Turkey
- Kimmel Cancer Center United States
- Shanghai Changzheng Hospital China (People's Republic of)
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Translation, ribosome protein S15a, polymerase chain reaction, complementary DNA, animal cell, Mice, SCID, Polymerase Chain Reaction, Western blotting, Mice, Ribosomal protein, cell growth, Northern, base pairing, Cloning, Molecular, In Situ Hybridization, Blotting, messenger RNA, Liver Neoplasms, article, unclassified drug, Up-Regulation, Gene Expression Regulation, Neoplastic, RNA isolation, liver carcinogenesis, priority journal, Liver, hepatitis B antigen, cell cycle, Western, carcinogenesis, amino acid, Cell Division, Ribosomal Proteins, Hepatocarcinogenesis, Hepatitis B virus, liver cell carcinoma, Carcinoma, Hepatocellular, DNA, Complementary, animal experiment, Blotting, Western, Molecular Sequence Data, 610, Mice, Nude, colony formation, Colony-Forming Units Assay, Hepatitis B Antigens, ribosome protein, SCID mouse, Animals, Humans, controlled study, human, Amino Acid Sequence, mouse, gene identification, Hepatitis B x antigen, nonhuman, RNA translation, Base Sequence, animal model, human cell, Carcinoma, Hepatocellular, molecular weight, Hepatitis b virus x antigen, Blotting, Northern, human tissue, Subtraction Technique, gene expression, Trans-Activators, Northern blotting
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