AbstractBackground and ObjectivesImaging of subcutaneous veins is important in many applications, such as gaining venous access and vascular surgery. Despite a long history of medical infrared (IR) photography and imaging, this technique is not widely used for this purpose. Here we revisited and explored the capability of near‐IR imaging to visualize subcutaneous structures, with a focus on diagnostics of superficial veins.Study Design/Materials and MethodsAn IR device comprising a head‐mounted IR LED array (880 nm), a small conventional CCD camera (Toshiba Ik‐mui, Tokyo, Japan), virtual‐reality optics, polarizers, filters, and diffusers was used in vivo to obtain images of different subcutaneous structures. The same device was used to estimate the IR image quality as a function of wavelength produced by a tunable xenon lamp‐based monochrometer in the range of 500–1,000 nm and continuous‐wave Nd:YAG (1.06 μm) and diode (805 nm) lasers.ResultsThe various modes of optical illumination were compared in vivo. Contrast of the IR images in the reflectance mode was measured in the near‐IR spectral range of 650–1,060 nm. Using the LED array, various IR images were obtained in vivo, including images of vein structure in a pigmented, fatty forearm, varicose leg veins, and vascular lesions of the tongue.ConclusionImaging in the near‐IR range (880–930 nm) provides relatively good contrast of subcutaneous veins, underscoring its value for diagnosis. This technique has the potential for the diagnosis of varicose veins with a diameter of 0.5–2 mm at a depth of 1–3 mm, guidance of venous access, podiatry, phlebotomy, injection sclerotherapy, and control of laser interstitial therapy. Lasers Surg. Med. 34:56–61, 2004. © 2004 Wiley‐Liss, Inc.