AbstractObjectives/Hypothesis:Many otologic disorders have been attributed to dysfunction of the tensor tympani muscle, including tinnitus, otalgia, Meniere's disease and sensorineural hearing loss. The objective of this study was to determine adequate stimuli for tensor tympani contraction in humans and determine markers of the hypercontracted state that could be used to detect this process in otologic disease.Study Design:Multiple types of studies.Methods:Studies included 1) measuring middle ear impedance changes in response to orbital puffs of air, facial stroking, and self‐vocalization; 2) measuring changes in stapes and eardrum vibrations and middle ear acoustic impedance in response to force loading of the tensor tympani in fresh human cadaveric temporal bones; 3) measuring changes in acoustic impedance in two subjects who could voluntarily contract their tensor tympani, and performing an audiogram with the muscle contracted in one of these subjects; and 4) developing a lumped parameter computer model of the middle ear while simulating various levels of tensor tympani contraction.Results:Orbital jets of air are the most effective stimuli for eliciting tensor tympani contraction. As markers for tensor tympani contraction, all investigations indicate that tensor tympani hypercontraction should result in a low‐frequency hearing loss, predominantly conductive, with a decrease in middle ear compliance.Conclusions:These markers should be searched for in otologic pathology states where the tensor tympani is suspected of being hypercontracted.