AbstractA reverse‐phase high‐performance liquid chromatographic (RP‐HPLC) method coupled with an ultraviolet detector was developed to determine triclosan which had migrated into foodstuffs from packaging materials. The method includes extraction with hexane, followed by evaporation to dryness and residue re‐dissolution in ACN 90%. Chromatographic separation was performed with a Kromasil 100 C18 column (15 cm×0.4 cm ID, 5 μm particle size) at 30°C and using ACN and water as mobile phases. Regarding recoveries, good results (higher than 83% and lower than 112%) were obtained for the three representative food matrixes selected (orange juice, chicken breast meat, and Gouda cheese).