AbstractAnalysis of Hibiscus sabdariffa seed oil from different seed collections (or cultivars), representing different growing areas, showed differences in the fatty acid pattern, especially with respect to linoleic (30.1‐37.45%) and epoxyoleic (trace to 5.3%). The results showed different values from those previously reported for the cyclopropenoid fatty acid, found to be malvalic acid (0.4‐2.0%), responsible for the positive Halphen response of the oil. It is now also established that the oil contains dihydrosterculic acid (1.0‐1.6%) which has been reported previously in other species of the genus Hibiscus. The results of the rest of fatty acids showed ranges for myristic 0.2‐0.5%, palmitic 17.4‐22.6%, stearic 3.9‐5.2% and oleic acid 34.6‐39.8%.