Competitive Protein Binding Assay for Piritrexim

descriptionPublicationkeyboard_double_arrow_right Article 01 Sep 1989 English Publisher:Elsevier BVJournal:Journal of Pharmaceutical Sciences, volume 78, pages 749-752 (issn: 0022-3549,

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Authors: J L, Woolley; J L, Ringstad; C W, Sigel;

doi: 10.1002/jps.2600780910

pmid: 2585269

Competitive Protein Binding Assay for Piritrexim

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Abstract

A competitive protein binding assay for piritrexim (PTX, 1) that makes use of a commercially available radioassay kit for methotrexate has been developed. After it is selectively extracted from plasma, PTX competes with [125l]methotrexate for binding to dihydrofolate reductase isolated from Lactobacillus casei. Free drug is separated from bound drug by adsorption to dextran-coated charcoal. Piritrexim is measurable over a range of 0.01 to 10.0 micrograms/mL in plasma with a coefficient of variation less than 15%. The limit of sensitivity of the assay is approximately 2 ng/mL. An excellent correlation between this assay and a previously published HPLC method was found. published HPLC method was found.

Related Organizations

Research Triangle Park Foundation
United States

Keywords

Iodine Radioisotopes, Pyrimidines, Folic Acid Antagonists, Humans, Antineoplastic Agents, Chromatography, Thin Layer, Binding, Competitive, Chromatography, High Pressure Liquid, Protein Binding

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