The retention behavior of cisplatin on a variety of stationary phases has been investigated using aqueous mobile phases modified by the addition of various electrolytes and methanol. Cisplatin is poorly retained on reverse-phase or silica columns but satisfactorily retained on chemically bonded or solvent-generated anion exchangers. The retention of the neutral complex on positively charged stationary phases is explained in terms of ion-dipole interactions and rationalized by the application of solvophobic theory. The use of solvent-generated anion exchangers for the analysis of cisplatin offers significant advantages over the chemically bonded system in terms of peak shape, column efficiency, and stability. By the use of column switching and off-line atomic absorption, solvent-generated anion exchange high-performance liquid chromatography (HPLC) is applicable to the determination of cisplatin in urine.