Fluorometric and GLC Analyses of Aristolochic Acid
Copyright policy )Fluorometric and GLC Analyses of Aristolochic Acid
Aristolochic acid is a natural product with possible implication in Balkan endemic nephropathy. A convenient fluorometric assay for the compound is described based on reduction to the lactam and measurement of the intensity of fluorescence. The limit of sensitivity was 0.05 mug/ml. A GLC assay is also described, based on flash methylation of aristolochic acid and its lactam using trimethylanilinium hydroxide. Conditions for optimum performance with a sensitivity limit of 1-5 mug/ml are described.
- University of Florida United States
Aniline Compounds, Chromatography, Gas, Lactams, Dioxolanes, Phenanthrenes, Plants, Nitro Compounds, Methylation, Aristolochic Acids, Fluorometry, Spectrophotometry, Ultraviolet, Chromatography, Thin Layer, Oxidation-Reduction
Aniline Compounds, Chromatography, Gas, Lactams, Dioxolanes, Phenanthrenes, Plants, Nitro Compounds, Methylation, Aristolochic Acids, Fluorometry, Spectrophotometry, Ultraviolet, Chromatography, Thin Layer, Oxidation-Reduction
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