Abstract Prior to 1967, liquid column chromatography (LCC) separations were not normally carried out in an optimum manner; for this reason, the technique was viewed as slow and inefficient (1–3). Consequently, other techniques such as thin-layer chromatography (TLC) and gas chromatography (GC) found favor. Within the last 5 years, LCC has undergone dramatic changes and now rivals GC in speed of analysis and column efficiency. The modernized technique is commonly known as high pressure liquid chromatography (HPLC).