A series of tests was conducted to obtain a profile of the toxicity of epichlorohydrin since this compound finds a number of industrial applications, including synthesis of some epoxy resins which may be formulated into certain biomedical materials. These included acute toxicity by the oral, intraperitoneal, dermal, and inhalation routes; toxicity to mouse fibroblast cells in culture; and hemolytic activity against rabbit blood. Irritancy activity was determined in rabbits by the intradermal, topical (dermal), ophthalmic, and muscle implant methods. Cardiovascular activity of the compound was investigated in rabbits. Alteration of pentobarbital sleeping time in mice was determined following administration by inhalation and intraperitoneal injection. Cumulative and subacute toxicity studies in rats were also conducted. In general, the compound proved to be quite toxic and very irritating in the systems employed.