
doi: 10.1002/jmv.2122
pmid: 11748670
AbstractUsing the RT‐PCR with primers that anneal to the 5′ and the 3′ extremities of the genome segments of bunyaviruses and internal primers that anneal to the S segment of Simbu serogroup viruses in a nested PCR it was possible to amplify the Oropouche virus (ORO) genome from the sera of three patients. These results show that this RT‐nested‐PCR is a useful tool for rapid diagnosis of Oropouche fever infections. J. Med. Virol. 66:139–142, 2002. © 2002 Wiley‐Liss, Inc.
Base Sequence, Reverse Transcriptase Polymerase Chain Reaction, Molecular Sequence Data, Simbu virus, Sequence Analysis, DNA, Bunyaviridae Infections, DNA, Viral, Humans, RNA, Viral, DNA Primers
Base Sequence, Reverse Transcriptase Polymerase Chain Reaction, Molecular Sequence Data, Simbu virus, Sequence Analysis, DNA, Bunyaviridae Infections, DNA, Viral, Humans, RNA, Viral, DNA Primers
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