Screening of supports for the immobilization of pectinmethylesterase from acerola (Malpighia glabra L)

descriptionPublicationkeyboard_double_arrow_right Article 17 Feb 2004 Brazil English Publisher:WileyJournal:Journal of Chemical Technology & Biotechnology, volume 79, pages 277-280 (issn: 0268-2575, eissn: 1097-4660,

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Authors: de Assis, S. A.; Fernandes, P.; Ferreira, B. S.; Cabral, JMS; Oliveira, OMMF;

doi: 10.1002/jctb.973

handle: 11449/33106

Screening of supports for the immobilization of pectinmethylesterase from acerola (Malpighia glabra L)

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Abstract

AbstractA partially purified extract of pectinmethylesterase (PME) from acerola fruit was immobilized on various supports: glass, celite, chrysotile, agarose, concanavalin A Sepharose 4B, egg shell, polyacrylamide and gelatin. In addition, reticulation with glutaraldehyde was assessed, as well as the use of gelatin in the presence of celite, glass and silica. The highest immobilization yields were obtained when the pectinmethylesterase was immobilized in concanavalin A Sepharose 4B (81.7%) and in gelatin‐water (78.0%). Copyright © 2004 Society of Chemical Industry

Country

Brazil

Related Organizations

Sao Paulo State University
Brazil
Universidade Lusófona
Portugal
Universidade Estadual Paulista - UNESP
Brazil
Instituto Superior de Espinho
Portugal

Keywords

immobilization, pectinmethylesterase, acerola, kinetics characterization

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