AbstractAU‐rich elements (AREs) in the 3′ untranslated region (UTR) of numerous mammalian transcripts function as instability elements that promote rapid mRNA degradation. Tristetraprolin (TTP) is an ARE‐binding protein that promotes rapid mRNA decay through mechanisms that are poorly understood. A 31 nucleotide ARE sequences from the TNF‐alpha 3′ UTR promoted TTP‐dependent mRNA decay when it was inserted into the 3′ UTR of a beta‐globin reporter transcript, indicating that this short sequence was sufficient for TTP function. We used a gel shift assay to identify a TTP‐containing complex in cytoplasmic extracts from TTP‐transfected HeLa cells that bound specifically to short ARE sequences. This TTP‐containing complex also contained the 5′–3′ exonuclease Xrn1 and the exosome component PM‐scl75 because it was super‐shifted with anti‐Xrn1 or anti‐PMscl75 antibodies. RNA affinity purification verified that these proteins associated specifically with ARE sequences in a TTP‐dependent manner. Using a competition binding assay, we found that the TTP‐containing complex bound with high affinity to short ARE sequences from GM‐CSF, IL‐3, TNF‐alpha, IL‐2, and c‐fos, but did not bind to a U‐rich sequence from c‐myc, a 22 nucleotide poly U sequence or a mutated GM‐CSF control sequence. High affinity binding by the TTP‐containing complex correlated with TTP‐dependent deadenylation and decay of capped, polyadenylated transcripts in a cell‐free mRNA decay assay, suggesting that the TTP‐containing complex was functional. These data support a model whereby TTP functions to enhance mRNA decay by recruiting components of the cellular mRNA decay machinery to the transcript. J. Cell. Biochem. 100: 1477–1492, 2007. © 2006 Wiley‐Liss, Inc.