AbstractObjectiveTourette syndrome (TS) is a chronic neuropsychiatric disorder. Its clinical manifestations are involuntary and recurrent muscle twitch, resulting in motor twitch and occurrence twitch. Traditional Chinese medicine has obvious advantages in treating TS. The aim of this study was to investigate the effects and mechanism of gastrodin on 1‐(2,5‐dimethoxy‐4‐iodophenyl)‐2‐aminopropane (DOI)‐induced TS in rats.MethodsTS model was induced by DOI. Behaviors in TS rats were detected. The striatum, serum inflammatory factors interleukin‐6, interleukin‐1β, and tumor necrosis factor‐a were detected by enzyme‐linked immunosorbent assay. Western blot technique was used to detect the expressions of TLR/NF‐κB and TLR/MAPK signaling pathways in the striatum.ResultsGastrodin can significantly improve behavioral changes of TS rats induced by DOI, reduce inflammatory factors in serum and striatum in TS rats, and inhibit activation of TLR/NF‐κB and TLR/MAPK signaling in striatum in TS rats.ConclusionGastrodin can significantly relieve the TS induced by DOI in rats. Its mechanism is related to the inhibition of striatal TLR/NF‐κB and TLR/MAPK signaling activation.