Suppressive subtractive hybridization libraries prepared from the digestive gland of the oyster Crassostrea brasiliana exposed to a diesel fuel water-accommodated fraction

descriptionPublicationkeyboard_double_arrow_right Article 13 Apr 2012 English Publisher:Oxford University Press (OUP)Journal:Environmental Toxicology and Chemistry, volume 31, pages 1,249-1,253 (issn: 0730-7268, eissn: 1552-8618,

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Authors: Karim Hahn, Lüchmann; Jacó Joaquim, Mattos; Marília Nardelli, Siebert; Tarquin Stephen, Dorrington; Guilherme, Toledo-Silva; Patricia Hermes, Stoco; Edmundo Carlos, Grisard; +1 Authors

doi: 10.1002/etc.1837

pmid: 22505345

Suppressive subtractive hybridization libraries prepared from the digestive gland of the oyster Crassostrea brasiliana exposed to a diesel fuel water-accommodated fraction

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Abstract

Abstract Diesel fuel can cause adverse effects in marine invertebrates by mechanisms that are not clearly understood. The authors used suppressive subtractive hybridization to identify genes up- and downregulated in Crassostrea brasiliana exposed to diesel fuel. Genes putatively involved in protein regulation, innate immune, and stress response, were altered by diesel challenge. Three genes regulated by diesel were validated by quantitative real-time polymerase chain reaction. This study sheds light on transcriptomic responses of oysters to diesel pollution. Environ. Toxicol. Chem. 2012;31:1249–1253. © 2012 SETAC

Related Organizations

State University of Campinas
Brazil
Universidade Federal de Santa Catarina
Brazil

Keywords

Animals, Hybridization, Genetic, Crassostrea, Biomarkers, Gasoline, Water Pollutants, Chemical, Environmental Monitoring, Gene Library

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