AbstractDisintegration fragments the loess body, causing erosion and the emergence of significant geohazards. The impact of vibrations on soil disintegration has been slightly documented; however, the contribution and mechanism of train vibration frequency in the disintegration of undisturbed loess remain unclear. In this study, train vibrations were monitored in situ, and the resulting vibrational parameters were used in loess disintegration tests using a customised vibration‐disintegration apparatus. The changes in the meso‐parameters of the disintegrated loess and aqueous solutions were quantified, and the microstructural differences in the residual loess after disintegration were compared under non‐vibrating and vibrating conditions. The results revealed that train vibrations in the loess progressively diminished with increasing distance from the track, with dominant vibration frequencies ranging from 17 to 49 Hz. Increasing the vibration frequency accelerated loess disintegration and enhanced the dispersion of the disintegrated fragments. Notably, the acceleration effect of disintegration was particularly pronounced in the early stages of increasing vibration frequency, and it tended to plateau above 15 Hz. The relationship between the vibration frequency and disintegration velocity (DV) of loess influenced by the initial water content can be expressed as a power function with variables. Vibrations accelerate loess disintegration primarily attributed to repetitive particle displacement and the vibrations of free water in the pores which lead to frictional damage to the weakly cemented structure and pore expansion. Higher vibration frequencies generate greater inertial forces and facilitate more frequent particle jumps, allowing the loess to reach the disintegration threshold conditions more readily than at lower frequencies. These findings provide theoretical value for the prevention and mitigation of water‐induced loess geohazards and land degradation in vibrating environments.