
pmid: 1748090
AbstractExperiments are described which elucidate some of the technical problems associated with the direct sequencing of polymerase chain reaction (PCR) amplified DNA. Sequencing primer purity, labeling methodology, and template preparation were explored. Conditions are presented for the routine sequencing of single‐ and double‐stranded PCR products.
Hypoxanthine Phosphoribosyltransferase, Base Sequence, DNA Mutational Analysis, Humans, Chromosome Deletion, Polymerase Chain Reaction
Hypoxanthine Phosphoribosyltransferase, Base Sequence, DNA Mutational Analysis, Humans, Chromosome Deletion, Polymerase Chain Reaction
| selected citations These citations are derived from selected sources. This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically). | 13 | |
| popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network. | Average | |
| influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically). | Top 10% | |
| impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network. | Top 10% |
