AbstractIn order to further meet the requirements of testing applications in various fields, a simple, rapid, and low‐cost testing method is demanded for on‐site testing. Electrochemical paper‐based analytical devices (ePADs) have attracted wide attention in many practical applications due to their excellent performance. ePADs combine the benefits of easy‐to‐use, low‐cost, electrochemical sensitivity and selective detection, as well as the advanced nature of pumpless equipment. In this paper, the latest development and new trend of ePADs are reviewed. First, several preparation methods of ePADs are introduced. We then reviewed examples of their use in various fields, particularly in point‐of‐care testing. Finally, the research of ePADs is summarized and prospected.