AbstractThe sugar oxidising enzymes glucose oxidase, glucose dehydrogenases (GDH) and cellobiose dehydrogenases (CDH) were co‐immobilised, in the presence of multiwalled carbon nanotubes, with osmium redox polymers. Under pseudo‐physiological conditions of 5 mM glucose, 150 mM NaCl, 37 °C, glucose oxidation current densities above 800 µA cm−2 are obtained from films containing an [Os(4,4′‐dimethyl‐2,2′‐bipyridine)2(poly‐vinylimidazole)10Cl]+ redox polymer, redox potential 0.1 V vs. Ag/AgCl, and either glucose oxidase or FAD‐dependant GDH. Current produced by, and stability of, glucose‐oxidising half‐cells is compared in 100 mM glucose, with films containing CDHs proving most stable. Such results show promise for development of glucose‐oxidising enzymatic fuel cells.