Identification of human cytotoxic ILC3s
Copyright policy )Identification of human cytotoxic ILC3s
Abstract Human ILCs are classically categorized into five subsets; cytotoxic CD127 − CD94 + NK cells and non‐cytotoxic CD127 + CD94 − , ILC1s, ILC2s, ILC3s, and LTi cells. Here, we identify a previously unrecognized subset within the CD127 + ILC population, characterized by the expression of the cytotoxic marker CD94. These CD94 + ILCs resemble conventional ILC3s in terms of phenotype, transcriptome, and cytokine production, but are highly cytotoxic. IL‐15 was unable to induce differentiation of CD94 + ILCs toward mature NK cells. Instead, CD94 + ILCs retained RORγt, CD127 and CD200R1 expression and produced IL‐22 in response to IL‐15. Culturing non‐cytotoxic ILC3s with IL‐12 induced upregulation of CD94 and cytotoxic activity, effects that were not observed with IL‐15 stimulation. Thus, human helper ILCs can acquire a cytotoxic program without differentiating into NK cells.
- Utrecht University Netherlands
- Amsterdam UMC Netherlands
- University of Amsterdam Netherlands
- Amsterdam UMC, location VUmc Netherlands
- Cancer Center Amsterdam Netherlands
Innate immunity, Cytotoxicity, Immunologic, Interleukin-15, Gene Expression Profiling, Immunology, innate lymphoid cells, Cell Differentiation, NK cells, Nuclear Receptor Subfamily 1, Group F, Member 3, Immunity, Innate, Cell Line, Interleukin-7 Receptor alpha Subunit, Killer Cells, Natural, Mice, IL-15, Gene Expression Regulation, IL-12, tonsil, Immunology and Allergy, Animals, Humans, Lymphocytes, NK Cell Lectin-Like Receptor Subfamily D
Innate immunity, Cytotoxicity, Immunologic, Interleukin-15, Gene Expression Profiling, Immunology, innate lymphoid cells, Cell Differentiation, NK cells, Nuclear Receptor Subfamily 1, Group F, Member 3, Immunity, Innate, Cell Line, Interleukin-7 Receptor alpha Subunit, Killer Cells, Natural, Mice, IL-15, Gene Expression Regulation, IL-12, tonsil, Immunology and Allergy, Animals, Humans, Lymphocytes, NK Cell Lectin-Like Receptor Subfamily D
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